FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305589
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12700.036.587961
TCTGTCG13300.035.7250678
GTCTTGC27050.031.4036831
TCGCTCT14400.029.496044
CTGTCGC17150.028.9644789
GTCTTAC12400.028.6903841
GGTATAC6900.028.4103531
TCTCGCT16550.027.4066642
TATACGG1607.1682036E-526.9967845
GTCTCAC34300.026.8826181
GTATAGT8000.025.4114821
CTCGCTC16600.024.7195743
GTGTTAG20050.024.623881
GTGTTAC12450.024.4929961
CGCTCTG16250.024.3663275
GGACCGA3551.6916601E-1024.3351296
GTATAAC6600.024.2014121
TAATACT10750.024.1083894
GTATTAT10550.023.3985231
GTATTGA9950.023.3501051
Produced by FastQC (version 0.11.5)