FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208408
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.038.8160748
GTCTCGC13200.038.209161
TCGCTCT14650.036.368554
CTCGCTC16850.030.7655353
CTGTCGC17150.030.230199
CGCTCTG16200.030.2217065
GTATTAG10500.030.1930331
TCTCGCT17150.029.8075352
GTCTTGC27800.029.2871461
TTATACT9400.027.5744274
GTAACGC1751.317028E-424.685683
GTGTTAG20800.024.5945221
GTCTTAC12600.024.5890241
GTTTCAC41350.024.5513553
GTCCTAT8350.024.1610011
GTACTGG13900.023.84441
GTCTCAC34550.023.5653421
GTATTAT10450.023.4426121
TCTGTCA36900.023.4167928
GGTTACG1851.9105636E-423.368071
Produced by FastQC (version 0.11.5)