FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684110
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14650.039.3141448
GTCTCGC14700.034.7995451
GTCTTGC31750.034.7200471
TCGCTCT16100.031.3034534
CTGTCGC18050.031.1109949
GTATAGA12050.026.3086261
CTCGCTC19050.026.0967643
TCTCGCT18200.025.732062
CGCTCTG18200.025.3179575
GTCTCAC38500.025.0770631
GTGTAAG19400.024.8831671
GTATTAT12150.024.313091
GTCTTAC14600.024.1810861
GTATTAA9900.022.5610371
TTGCTCT44400.022.5398944
GTACAGA20950.022.3543421
GTCTAAC9050.022.2916411
GTGTAAA22000.022.2699281
GTATTAC8100.022.2375831
TAATACT9850.021.9283054
Produced by FastQC (version 0.11.5)