FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684110
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14850.037.3370978
GTCTTGC31450.029.7790811
CTGTCGC19650.028.9494869
GTCTCGC13650.028.5003191
GTGTTAG20450.026.0691591
GTGTAAC10600.025.8265481
GTATTAG11500.024.4317931
GTCTTAC13350.024.283981
TCGCTCT17300.024.1395244
GGTATAC7100.023.3377041
GTATTAA11100.022.7160951
GTACTAA8000.022.5132711
TTGCTCT44300.022.4296324
GTCTCAC39550.022.2229611
GTATTAT11050.022.1669141
TCTGTCA41100.021.9000178
CTCGCTC17950.021.6608833
GTGTAAG17650.021.6331521
GTATAAT11050.021.5149461
GTACCGC2353.8984195E-521.459461
Produced by FastQC (version 0.11.5)