FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225608
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.040.9816178
GTCTCGC13400.036.559011
CTGTCGC16150.036.108819
TCGCTCT14650.031.4516244
GTCTTGC28250.029.3271351
GTATTAC7500.028.8170971
TCTCGCT16200.026.2377912
CTCGCTC16150.024.9807683
GTATTAT10450.024.8185541
CGCTCTG15700.024.762525
GTTTCAC43250.024.3196333
GTCTTAC13350.024.2840731
GTCTAAC6850.024.1895351
GTATTAA8700.024.014251
GGTATAC6850.023.1378171
TACACTG17750.023.1194345
TACACCG2852.9769035E-722.7351785
GGGTTTC48600.022.6801241
GCGTAAT1600.002257689822.5133611
GTCTAGC13200.022.3769151
Produced by FastQC (version 0.11.5)