FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225608
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.045.9256068
GTCTCGC13550.038.2761651
CTGTCGC17300.036.211529
GTCTTGC28250.035.4430921
TCGCTCT15500.032.5161824
TCTCGCT17050.028.29332
CTCGCTC17650.027.739423
GTCTTAC11950.026.5228391
GTACTAT6950.025.9113731
GTATTAG9600.025.5119081
GTATTAC7750.025.0955851
CGCTCTG16450.024.9483685
GTCTCAC32350.024.7163261
GTGTTAG19500.024.0112061
TTGCTCT39300.023.2672144
GTATTAT9000.023.2108331
GTATTGA7950.022.652081
GTCCTAC9600.022.5105081
GTCTAGC11600.022.355261
CTTGCTC37950.022.1976663
Produced by FastQC (version 0.11.5)