FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468712
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.038.31158
GTCTCGC12900.037.296391
TCGCTCT12850.036.4391254
CTGTCGC13950.034.0590939
GTCTTGC29050.028.606931
CTCGCTC15200.027.9780083
TCTCGCT15900.027.6529242
GTGTAAA19400.026.3032191
GTACCGG3108.076313E-1025.866851
CGCTCTG16150.024.961615
GTGTAAC9950.024.1770551
GGTATAC5150.024.0632481
GAGTGCA76950.024.042616
GGCTAGA20550.023.7670651
GGAGTGC69500.023.7196225
GTGTTAG21900.023.6334321
GTCTTAC13100.023.3717141
GTGTATA15750.022.6792181
TTATACT9650.022.3950314
GTACGTC1952.7217087E-422.1781333
Produced by FastQC (version 0.11.5)