FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468221
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13350.044.2175838
CTGTCGC16650.038.0479139
GTCTCGC14800.035.001241
TCGCTCT15650.032.2285464
GTCTTGC28900.030.0424881
CTCGCTC17950.027.714373
TCTCGCT18900.026.3213182
GTGTTAG23700.026.1672061
GTGTAAC10950.025.3195691
CGCTCTG19400.025.2319495
GTACTAT7450.024.483331
GTCTCAC38400.024.130111
GTCTTAC11850.024.012261
GTATTAT10700.023.8655281
GTGTAGG17250.023.685671
GTATTAG10250.023.4896661
TAATACT11700.023.4020984
GTATACG2502.1597625E-623.3473051
GTTTCAC53750.022.8029373
GCGTAGA3203.17159E-822.8001021
Produced by FastQC (version 0.11.5)