FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875016
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12750.049.7242931
TCTGTCG12050.049.591558
TCTCGCT15150.041.3716472
TCGCTCT14600.040.953514
CTGTCGC15400.039.7388089
CGCTCTG15850.036.3393635
CTCGCTC16450.035.034023
GTCTTGC28250.031.367711
TTCACCG8000.027.8989775
GTCTTAC11150.027.783681
GTCTCAC31800.026.9597591
GTGTTAG19650.026.0310651
GCGGTAC1751.3115522E-424.7007061
TCACCGT10150.024.1173446
TGTACGG2401.690174E-624.0145762
GTATTAC7550.023.8555371
TCTGTCA34950.023.2781188
CTTGCTC34500.023.1776733
GTATTGC6250.023.0539911
CTGTTGC30400.022.4991769
Produced by FastQC (version 0.11.5)