FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875016
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11500.044.5109331
TCTGTCG12400.039.4844938
TCGCTCT13250.036.408124
CTGTCGC14100.034.2133069
CGCTCTG14950.031.3048425
GTCTTGC25700.030.8578571
CTCGCTC15300.029.6475243
TCTCGCT15700.029.3497562
GTGTTAG17300.028.3379861
GTATTCG2105.242291E-727.4648271
GTACGGG1856.080074E-627.2792531
TCTATCG2156.5124004E-726.7911198
CCGTGTT9300.025.5487889
GTCTTAC12200.024.8196491
GTCCTAC8450.024.742721
GTATAAG8550.024.4533331
GTCTCAC31900.023.7303851
TAATACT9500.023.4951064
GTACAGA14900.023.225291
GTATTAA9750.022.9225671
Produced by FastQC (version 0.11.5)