FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037339
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15350.039.8627628
GTCTCGC16000.035.086321
TCGCTCT18950.034.60264
TCTCGCT18800.032.591112
GTCTTGC35550.032.4029541
CTGTCGC19800.032.3579989
CGCTCTG20300.029.7871745
CTCGCTC20100.029.0487273
GTGCTAA13950.025.0861571
GTTTCAC53850.024.0948873
TTCACCG12150.023.6989865
GTATTAG13300.023.571321
TTGCTCT49650.023.5110654
GGGTTTC59850.023.5104141
GTATTAT11300.023.2268941
GTGTTAC15150.023.0991331
CCGTTCG2202.3567489E-522.90519
GTGTATA17200.022.8892961
GTACAAG15300.022.8726731
ACCGTGT12400.022.6412148
Produced by FastQC (version 0.11.5)