FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037339
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15850.043.0695881
TCTGTCG15600.042.9167988
GTCTTGC34900.036.2072831
TCGCTCT20350.034.667724
CTGTCGC21850.034.265019
CTCGCTC22550.031.2855053
TCTCGCT21950.029.8464012
CGCTCTG22700.027.9078145
GTGTTAG23300.027.4282991
GTATAAG9450.026.8972781
GTACTAG5700.026.7557141
GTCTCAC38850.025.2356721
GTCTTAC14900.024.370021
GTGTTAC13150.024.2995951
GTATTAG12700.024.0169411
TTGCTCT50050.023.73254
GTGTAAA20550.023.3240641
TCGACCG1550.001885364423.222419
GGGTTTC60450.022.9461151
GTTTCAC53400.022.7829023
Produced by FastQC (version 0.11.5)