FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937302
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14150.046.8049438
GTCTCGC16150.041.5436251
CTGTCGC17450.036.303429
TCGCTCT18200.034.4398544
GTCTTGC33800.031.2853031
TCTCGCT20400.029.6801992
CTCGCTC20700.026.8125483
CGCTCTG19500.026.2106975
GTACTAG6800.024.6665251
GTCTCAC40800.024.1302951
GTGTTAC14250.024.0531561
GTGTTAG27000.024.0389391
GTATTAC9850.023.692061
GTGTAGG20700.023.6044311
GTATTGA12900.022.613061
GGATACG3553.8635335E-922.5971361
GTCTTAC16250.022.4391141
GGGTTTC59250.022.2781581
GTTTCAC54600.022.0465973
GTATTAT13050.021.7943131
Produced by FastQC (version 0.11.5)