FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448423
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14950.038.3729321
TCTGTCG15100.035.279668
TCGCTCT17000.033.8764654
GTCTTGC29250.031.0329631
TCTCGCT19250.029.5454882
GTGTAAC9200.029.204721
CTGTCGC19000.028.7958349
CTCGCTC20600.027.9563063
CGCTCTG19000.026.5224785
GCGTAGT3851.6370905E-1124.5200711
GTCTAGC14050.022.741311
GTCGAAC1600.00215589722.6928541
GTATAGT8000.022.6928541
GAGTGCA80600.022.6865446
TATACTG9850.022.6566185
GGAGTGC76100.022.5144825
TTATACT8700.022.3409464
GTGTTAG20850.022.2901521
GTATTAG8800.022.2802581
GTGTAGG18300.021.8248231
Produced by FastQC (version 0.11.5)