FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705199
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11250.034.845651
TCTGTCG11000.034.032258
CTGTCGC15200.026.9967379
GTCTTGC22250.026.7541481
GTCTCAC28700.026.3062381
CTCGCTC15500.026.0097583
TCGCTCT15050.025.8307634
TCTCGCT15550.025.464572
GCGTAGT2901.17597665E-825.0327931
GTGTAGG12050.024.097961
CGCTCTG15000.023.0372145
GTGTAAA12900.022.510111
CGTAGTG3203.6514393E-822.498532
GTATTGG7600.021.9695721
TTACACT10600.021.7332214
GTGTTAG15050.021.2238161
GTATTTA9050.020.8560521
TACACTG14650.020.6391415
GTATAAT6350.020.578141
GTGTAAG11050.020.3660471
Produced by FastQC (version 0.11.5)