FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803276
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12250.050.563331
TCTGTCG11050.044.9542778
TCGCTCT12600.041.1683924
TCTCGCT14650.039.850652
CTGTCGC13850.036.385849
CTCGCTC15250.034.5017433
CGCTCTG16150.032.0932125
GTCTTGC23600.031.8037321
GTGTTAG17650.030.9648821
GTTCCGA1606.5992754E-527.326511
GTATTAC5750.026.6136421
GTCTCAC29150.024.9985221
CTTGTCG2901.285116E-824.8248259
GTATTAG6850.024.467531
GTCTTAC10450.024.406451
TGTTAGC17450.024.3693872
TTGCTCT31950.024.353134
GTTAGCC15450.023.7919333
GTACAGA12950.023.6337361
GTATTAT6550.023.3631211
Produced by FastQC (version 0.11.5)