FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803276
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.039.6234058
GTCTTGC22550.036.056161
GTCTCGC12150.035.849491
TCGCTCT12900.034.600724
CTGTCGC16950.031.854799
CTCGCTC14800.028.699443
GTGTTAC10000.028.3121341
GTGTTAG15050.027.4945351
TCTCGCT15050.026.789092
CGCTCTG13400.025.7881155
GGTATAC4250.025.6218411
GTGTAAA14050.025.3179041
ATGGCGT12800.025.3096266
GTATAGG6950.025.0688531
GTATTAG7750.024.3545251
GTCTTAC10000.023.956421
GTTTCAC37600.023.933453
GTATTGA7950.023.7418331
TTCACCG7900.023.6935125
GGAGTGC66000.023.451885
Produced by FastQC (version 0.11.5)