FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078185
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12100.047.5960731
TCGCTCT13550.040.4120864
TCTGTCG11450.040.2394038
TCTCGCT14750.036.1603052
CTGTCGC14500.033.7612049
CTCGCTC15250.033.556823
CGCTCTG16800.031.7076595
GTCTTGC27150.031.6841161
CATACCG1255.3759816E-428.7939835
GTATTGA9550.026.7173941
GTGTTAC11700.026.1693441
GTATTAG8700.025.9759731
GGGTTTC46000.024.7227151
TTGCTCT37200.024.4042024
GGTATAC5100.024.3001041
GTACTAG6100.023.9017431
CTGTTGC32050.022.9112729
GTCTCAC33050.022.7193131
GTTCTAC8350.022.6994971
GTTTCAC42550.022.3597373
Produced by FastQC (version 0.11.5)