FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078185
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.043.3628238
GTCTCGC12350.037.6076741
GTCTTGC25850.037.0575641
CTGTCGC18100.033.013559
TCGCTCT13850.029.1078264
TTGCTCT35650.028.6747994
TCTCGCT15200.027.9452632
CTCGCTC15200.027.4698283
GGTATAC6150.027.1403961
GTCTTAC12600.027.0701581
GTATTAG9400.026.2491071
GGGTTTC45000.025.8030431
CGCTCTG14850.025.6946145
GTGTTAT10200.025.6133141
GTATAAT8850.024.6003571
GTGTTAG17750.024.1222111
GTCTCAC33800.024.0472151
GTTAGCC13900.023.8239923
ATGGCGT11300.023.5730636
GTTTCAC41700.023.478723
Produced by FastQC (version 0.11.5)