FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351288
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.038.9141548
GTCTCGC10850.035.687581
GTCTTGC20700.032.470461
TCGCTCT11400.030.9435834
CTGTCGC12950.030.5754079
TCTCGCT12850.030.2550332
GTATTAA7100.027.7828251
GGTATAC4900.026.837831
GTACTAG5450.026.8104721
GTATTAG7450.026.4775921
CTCGCTC13700.025.7508623
CGCTCTG13450.024.621525
GTGTATA11500.024.1410831
GTGTTAC9200.023.8234351
GTATAAC4002.3646862E-1123.7440261
CTGTTGC25300.023.617669
GTGTAAC6600.023.2458971
GTCTAGC10400.023.1820351
GTATAAT7050.022.798411
GGGTTTC39700.022.6352651
Produced by FastQC (version 0.11.5)