FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351288
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10400.037.513551
GTCTTGC20500.030.521841
TCGCTCT10550.029.3417594
GTCTTAC8650.028.9339961
TCTCGCT12350.028.56272
GGTATAC3950.027.9537321
TCTGTCG9800.027.914368
GTGTTAG15950.026.30631
GTATTAC5900.026.20071
CGCTCTG12200.025.371795
CGTTTCG1751.317963E-424.6826959
GTACGGA2401.4011603E-624.5371631
GTATAAT7550.024.3746641
GTATATA9450.024.1476841
GTATTAT8350.023.8025191
GTGTAAC7850.023.443151
TTGCTCT30450.022.9326674
CTCGCTC13200.022.9058023
CTTGCTC29550.022.9002653
GTCCTAT6500.022.6496891
Produced by FastQC (version 0.11.5)