FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596581
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.036.1809238
GTCTCGC10100.035.6958271
CTGTCGC11500.030.678289
GTCTTGC21000.029.8723071
TCGTACA1703.1811305E-629.6470744
GTATTAG8050.028.6630841
TCGCTCT11700.026.4615524
TCTCGCT13050.025.3793222
GTGTTAT9850.024.889231
GTGTACG2901.2636519E-824.8639891
GTATCGC1500.001543162424.0351891
CTCGCTC13200.023.454563
TGTCGAC1550.001883575923.2258197
GTATAGA6300.022.8906561
GTATTAA7300.022.7181911
GTCTAAC6050.022.6447241
GTATTCG1952.7146586E-422.186331
GTATAAC4900.022.0731331
GGGTTTC44200.021.6969221
ATCGTAC2003.2535498E-421.600013
Produced by FastQC (version 0.11.5)