FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947660
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16450.038.102491
TCTGTCG15650.036.8044668
TCGCTCT17450.035.9189644
CTGTCGC18250.033.928179
CTCGCTC19050.031.7671893
TCTCGCT20700.030.6275542
CGCTCTG19700.030.3344945
GTCTTGC35650.028.898581
GTATTAA12800.028.1423261
GTATTAG13350.026.4432471
GTCTCAC43050.025.6046161
GTGTTAG25450.025.4773771
GTATAAT11550.024.3266661
GTGTTAC15350.022.9978751
GTGTAAC11450.022.0222911
GGTATAC7700.021.5197431
GTCTTAC15600.021.243851
GTCCCGT5101.8189894E-1221.1895181
GTGCTAC14150.020.8750441
TCTGTCA50300.020.7551028
Produced by FastQC (version 0.11.5)