FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599604
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATGTATATCTCGTATGCCGT42560.16371724308779337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC22050.035.287671
TCGCTCT12400.029.6311134
GTCTCGC11300.028.690751
GTACCGG1856.1123283E-627.2605231
TCTGTCG9950.026.0492258
GTATCGG1400.001037245725.7305951
CTCGCTC13250.025.5553283
CGTCAGA3251.6007107E-924.3846841
GTCTTAC8350.024.1590231
TCTCGCT13400.023.6567862
CGCTCTG14950.022.6345625
CTGTCGC13100.022.5334639
GTATAAG6250.021.9018821
CTTGCTC27850.021.729693
TAATACT6300.021.7276384
GTGTAAC5650.021.6774561
TTGCTCT31950.021.6471084
GTCTAAC5400.021.3468631
CTGTTGC26050.021.004939
GTCTCAC27900.020.9164831
Produced by FastQC (version 0.11.5)