FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599604
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11700.036.9652521
TCTGTCG10550.034.80598
GTCTTGC22250.028.5089321
CTGTCGC13150.028.4716579
TCTCGCT14700.027.4282152
TCGCTCT13150.026.2815284
GTCCGTA1400.001034178525.743661
GTCTCAC28250.025.260681
CTCGCTC14850.024.7274253
GTATAAG7050.024.5386371
GTGTTAC8550.024.4489481
GTATAGG4850.023.779711
CCGTTCG1902.2996581E-422.7369829
GTGTTAT6950.021.7802471
GCGTTAG2003.2285554E-421.6246741
GTATAGA5700.021.4982131
TTGCTCT30650.021.3769664
GCGTTGT2755.4835928E-620.9693811
GTGTTAG14900.020.802261
GTGTAAG8850.020.3622151
Produced by FastQC (version 0.11.5)