FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424173
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18300.045.6380738
GTCTCGC18150.038.497851
CTGTCGC21850.037.2346579
TCGCTCT20550.036.454664
GTCTTGC39850.032.8991281
CGCTCTG21900.031.2320045
TCTCGCT23250.028.5040262
CTCGCTC22900.027.9953583
GTATTAC10750.027.4736731
GTGTAAC13100.025.2946031
GTATTAG15800.024.619431
GGTATAC8800.023.7386841
TTGCTCT56850.023.4408174
GTGTAAA25800.022.6155221
GTGTTAG31050.021.8075871
CTTGCTC55200.021.7925913
TCTGTCA53300.021.7479868
GTGTTAC16650.021.6320191
GTCTCAC50000.021.322251
GTATAGA14250.021.2312581
Produced by FastQC (version 0.11.5)