FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424173
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG19100.042.596588
GTCTCGC19450.037.8051071
CTGTCGC23500.036.765729
TCGCTCT23350.034.2267574
GTCTTGC40600.031.783151
CGCTCTG24450.029.447665
CTCGCTC25050.029.3171733
TCTCGCT24500.028.799812
CGTTTCG2354.6038622E-827.574299
GTGTAAT16650.023.8132321
GTCTTAC18350.023.5713841
GTGTAAC13300.022.7649971
TTGCTCT57100.022.4446874
GTCTCAC48650.022.0786911
GTACTAA9550.021.8909451
GTATCGC1650.002689537421.8451981
TATACCG1650.002709028321.8180395
GTATTAG15650.021.6497781
GTGTAAA27850.021.4843791
GTATTAT15200.021.3421841
Produced by FastQC (version 0.11.5)