FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984520
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12750.042.9785841
TCTGTCG11600.040.9659468
TCGCTCT14550.036.124094
CTGTCGC15350.033.7723439
GTATTAC6750.033.1136131
CTCGCTC15150.032.7924233
TCTCGCT16100.031.3036272
CGCTCTG15750.030.6288915
GTCTTGC26200.028.3455311
GCGTTAC1607.093334E-527.0383341
GTCTTAC12200.026.0040821
GTATTAA8200.023.7409741
GTATACG2151.948543E-523.4751431
GTGTTAG19050.023.4663411
GGATCGT3753.3651304E-1023.0402438
GTGTTAC13150.023.0288471
GTATAAC5700.022.7691251
GGGTACG1902.277879E-422.7691231
TCTATCG1600.002265313622.5002358
GGTCGTA1600.002265754622.4994812
Produced by FastQC (version 0.11.5)