FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135869
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16150.040.1226928
GTCTCGC18650.036.6976241
TCGCTCT20700.033.062124
CTGTCGC19950.032.8411649
GTCTTGC36200.030.0511821
TCTCGCT22050.029.7321242
CGCTCTG22000.028.7991755
CTCGCTC22650.028.3073483
GTATTAC9300.025.5637281
GTATAGA12800.025.3276961
GTATTAA13700.022.6121061
GTATAAT14100.022.4815751
GTATTAT13600.022.2486461
TATACTG12500.021.8873735
GTCTTAC18000.021.6129681
GTATAGG9350.021.5744421
GTCTCAC47200.021.5213871
GTATAAG13000.021.058791
GTGTAGA20400.020.8360331
GTGTTAC16700.020.7070351
Produced by FastQC (version 0.11.5)