FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4135869
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16950.035.682068
GTCTCGC18150.033.765341
GTCTTGC37850.033.335041
CTGTCGC20600.031.4568779
TCGCTCT20450.029.5747474
CGCTCTG21700.027.2075355
TAATACT13300.027.067834
TCTCGCT22350.026.737772
CTCGCTC21400.025.9066963
GTATTAA14100.025.5669941
GTATAAC7900.024.6414051
GGTATAC8650.024.1718961
GTGTTAC17800.023.4929081
GTGTAAT16600.022.1508731
GTGTTAG26200.022.0149381
TTGCTCT57750.020.6962284
GTATTAG15100.020.5314831
GTATTAC11300.020.4173951
CTTGCTC54150.020.0776823
TCTGTCA52000.020.0772848
Produced by FastQC (version 0.11.5)