FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980940
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14650.037.8420338
CTGTCGC18700.033.1114549
GTCTCGC16700.032.358481
TCGCTCT19300.028.744874
GTCTTGC34200.028.6520791
CGCTCTG20450.024.6448465
CTCGCTC19950.024.1968523
TCTCGCT20300.024.1354962
TTATACT12300.024.016284
GTACCGA1550.001875930723.2424371
GTCTTAC14700.023.036891
GTATATA14150.022.4047241
GTCTCAC43850.021.853721
GTCTAGC15850.021.8200281
GTATTAG11850.021.2810481
GTGTTAC14500.020.8701061
GTGTAAG16650.020.771621
GTGTAAA23100.020.5861591
GTACTAT9100.020.5861571
GCGTTAC2557.304379E-519.7788581
Produced by FastQC (version 0.11.5)