FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980940
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17100.039.1583488
TCGCTCT20150.038.9478454
GTCTCGC19450.037.4337431
CTGTCGC20550.033.6354269
CTCGCTC21800.033.357763
CGCTCTG21950.030.8336815
TCTCGCT24450.030.3308752
GTCTTGC38050.029.7444881
GTATTAT11550.026.2137281
GTACTAG7600.025.6101231
GTATTAC8500.024.5946451
GGTATAC6700.023.6706051
GTCTCAC44900.023.4405611
GTGTTAC13300.022.7645531
GTATTAG13900.021.781911
TTGCTCT53050.021.3081784
GTGTTAG25450.020.677431
GTACAGA19600.020.5965021
GTCTAGG18450.020.3174171
GTGTAAC11400.020.2351571
Produced by FastQC (version 0.11.5)