FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326663
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.044.3020328
GTCTCGC9250.034.758191
CTGTCGC14600.032.05079
GTATTAC5300.031.7101421
TCGCTCT10350.028.5180954
GTCTTGC22250.028.243251
GTATTAT7350.027.8366451
TAGTACG1050.00798029927.4250664
TCTCGCT11200.026.9988732
GTGTTAC10350.026.8280721
GTCTTAC9200.026.2103211
GGTATAC4200.026.0968551
GTGTTAG14850.025.5872211
GTATTGA7800.025.2938791
CTCGCTC11500.024.4168953
TCGAACT14500.024.3279259
GTCTCAC26150.024.0310631
GTACGGG1902.0807884E-423.0751151
TACCCGT1902.3016431E-422.7339385
GTATAAG8100.022.552841
Produced by FastQC (version 0.11.5)