FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326663
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.041.7145968
GTCTCGC9800.041.3125081
TCGCTCT11950.033.734344
TTACGGT1751.0752228E-732.908194
CGCTCTG12850.030.2505595
TCTCGCT14100.029.1003722
GTCTAAC6200.028.4947261
CTCGCTC13100.028.0247463
CTGTCGC15050.027.7441629
TAACACT11250.027.5149084
GTCTTGC21700.026.4593891
GTACAAG8750.026.0794561
GTCTCAC26600.024.3526361
GTGTTAG16350.024.3120141
GTCCGGG4850.024.2841641
GTATTAG8500.024.2484531
TCCGTAC1500.001558207723.9950453
GTGTTAC10250.023.699271
GTGTAAT10450.023.2456971
ACCGATC1550.001884844923.2230058
Produced by FastQC (version 0.11.5)