FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652554
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11250.037.0496441
TCTGTCG10950.034.844418
GTCTTGC23100.032.6052061
TCGCTCT12600.031.9954974
TCTCGCT14600.028.1082822
CTCGCTC13600.028.058053
GTATTAG9300.027.5198961
CGTAATA1607.164138E-526.9987432
CGCTCTG14550.026.2231145
CTGTCGC15000.024.4770159
GTATTAA7850.024.2195131
GTGTATA13650.023.0354921
GTATTAT7900.022.2149751
TCGATCT10050.022.206259
GTGCTAC8250.022.1588761
CTCGATC9750.022.1507288
TTGCTCT33550.021.8866374
TCTCGAT9900.021.8151117
GTGTTAC12150.021.6664561
GTATTAC8150.021.5335331
Produced by FastQC (version 0.11.5)