FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898647
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8100.045.7760281
TCGCTCT8750.038.6724134
CTCGCTC10150.034.7569353
CGCTCTG9650.034.3195845
TCTCGCT10850.033.1912272
TCTGTCG5900.028.0664378
GTCTTAC7300.027.8861241
GTATAAG6400.026.127681
GTCTTGC15650.024.62151
GTCCGCG1851.7970998E-423.5793861
GTATAAC3803.5106495E-1022.9588761
GTATTGC4850.022.4854981
GTGTAAA9750.022.3701861
CTGTCGC9000.021.5989539
GTATTAT5950.020.7723161
GGCTGGA55100.020.583821
GGAGTGC36050.020.370725
GAGTGCA38600.020.3311586
GTATAGA5403.6379788E-1220.1953091
GTCATAC4001.582157E-819.9933551
Produced by FastQC (version 0.11.5)