FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219851
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.042.3476688
GTCTCGC9200.034.179991
GTCGAAC1102.3165217E-433.240581
GTCTTGC19900.032.3385731
TACTCCG2051.2212695E-831.6051065
CTGTCGC13750.029.8442299
TCCGTAC1306.758152E-427.688863
GTGTATA12750.026.957461
TCGCTCT11550.026.1779694
TCTCGCT12150.025.4777362
CTCGCTC11900.025.4086023
TTGCTCT26750.024.4898554
GGTATAC5550.023.7176061
CGCTCTG11250.023.6765175
GTGTTAC9300.023.5900881
TAATACT7150.023.1574334
GTATAAC4750.023.0934561
GTTTCAC33650.022.6777113
GTCTCAC27150.022.6256331
GGGTTTC37950.022.5457861
Produced by FastQC (version 0.11.5)