FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099729
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.037.600428
GTCTCGC8700.033.6262441
GTATTAA6650.030.7945581
TCGCTCT10200.028.9362014
GTCTTGC19050.027.642361
GTGTTAG12800.027.4264051
CTGTCGC11850.027.3390249
TCTCGCT10300.027.2587432
CGCTCTG10200.026.8195555
CTCGCTC10500.026.7395323
CGTTTCG1358.424139E-426.6639849
GTATAAC4400.026.5953031
TCCGTAC1400.001041778225.711093
GTGTTAT7400.025.696811
GTGTACG1751.1838944E-425.075571
GTATTAG7100.024.7223931
GTTACAC5950.024.1986713
GACCCGT1801.5957664E-423.9964456
GTATAAG6550.023.4485281
GTGTTAC8750.023.4038661
Produced by FastQC (version 0.11.5)