FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370523
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7900.038.2732548
GTCTTGC21200.030.3545911
GTATTAG8500.028.390471
CTGTCGC11850.027.945559
GTCTCGC10600.025.5254521
GTACACG2651.267872E-724.8355751
GTATAAT7250.024.207551
TCGCTCT10750.023.438664
GTATTAT8250.023.0460741
GGTATAC4800.022.8521791
GTGTAAA14550.022.6165891
TAATACT6450.022.3225334
GTGTAAT9700.021.8627011
GTATAAA10450.021.6931691
GGCTAGA13500.021.6672521
GCGCAAT5400.021.3304219
TTGCTCT31550.021.2205564
GTATAGG6900.021.1962241
GTGTTAG14000.020.8934211
GGAGTGC48400.020.8236055
Produced by FastQC (version 0.11.5)