FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370523
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9550.034.701451
TCGCTCT10500.030.1658694
TCTGTCG10350.029.9087928
GTCTTGC20000.027.616571
GTATTAC6050.026.7797051
TCTCGCT11750.026.344082
GTATTAG7600.025.1940651
CGCTCTG10550.024.5636755
GTCTTAC7600.024.2250611
GTGTTAG14000.023.6713491
GTGTAAC6600.023.4322411
GTCTCAC24000.023.320661
CTCGCTC12150.023.1068523
CTAGCGT1902.3025423E-422.732653
GTATAAT8750.021.8828451
GTTTCAC32400.021.5515843
TTACACT10050.021.4885794
CAACGGC2353.920999E-521.4428584
GTTCTAC7350.021.0411971
TTCACCG6200.020.8989335
Produced by FastQC (version 0.11.5)