FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905620
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.039.115658
TCGGTAC951.0729699E-437.889183
GTCTCGC7700.035.2292441
GTCTTGC16500.034.657961
TCCGTAC1453.664596E-529.7887333
CTGTCGC13400.029.5479039
TCGCTCT9450.027.4252684
GTATACG1357.574272E-427.1536731
CGGTACT1358.424828E-426.6634564
TTACACT9400.026.0394174
TACACTA4150.026.0195945
GTGTTAG12750.025.3008351
GTACTAC3201.0913936E-925.2020031
GTATTAA6250.024.6338121
CGCTCTG10750.024.1074585
ACCGTGT5400.023.9964798
GTATAAC4002.3646862E-1123.8273491
GTCTTAC6500.023.6863571
GGGTTTC29350.023.230961
GTACTGC6650.023.1520791
Produced by FastQC (version 0.11.5)