FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242663
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC19350.042.342741
TCTGTCG9850.041.6583148
GTCTCGC8700.040.361181
TCGCTCT9550.039.9517254
GTATTAC4750.036.9623451
CTGTCGC13000.033.2254569
TCTCGCT10500.032.910492
CTCGCTC10600.031.2416783
GTCTTAC6950.028.4197861
CGCTCTG12000.027.5955855
TTGCTCT27800.027.4488474
GTATTAT5150.026.9890921
GTATTAA5350.024.6127781
CTTGCTC29150.024.450013
TAATACT6100.023.6027814
TTTACGC1550.001884765923.2231273
TCTTGCT33350.023.0977862
GTATTAG7000.022.9914591
GTCTCAC24200.022.9741861
GTGTTAG15000.022.4340881
Produced by FastQC (version 0.11.5)