FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242663
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG450.00991617747.9898229
GTCTCGC10350.037.034051
TCTGTCG10350.034.7752388
GTCTTGC21100.033.5372161
TCGCTCT10600.031.9191864
ACCGTGT7000.027.7655438
TTGCTCT28550.027.7361624
CTCGCTC12400.027.2857533
CGCTCTG13400.026.8593795
GTGTAAT8950.025.5315321
TCTCGCT12150.025.4772172
TTCACCG6300.023.9943795
CTGTCGC15800.023.691189
GTTTCAC29600.023.5906473
ATACCCG1851.9217806E-423.3474434
GTCCTAC6700.023.1037641
CCGTGTT6950.022.7865379
CTTGCTC27900.022.7058753
TACCCGT1600.002268362322.494735
GTATTAG6900.022.4340881
Produced by FastQC (version 0.11.5)