FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373556
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.036.542738
GTCTCGC9350.029.7005961
GTCTTGC22600.028.455581
GTATTAG7750.028.2886851
GTATTAT6750.028.1489891
CTGTCGC13650.026.8976219
GTATAAC3657.2759576E-1226.0281721
GTATTAC5950.025.7926241
TCGCTCT11300.025.4828934
ACCGTGT7450.025.1237378
GTATTGA7600.025.0007441
TCGGACG1450.001278562524.8238534
TTGCTCT30250.024.7499944
GTGTTAG14900.024.523191
GTTCGGT1500.001427184724.3597011
GTCTCAC25250.023.4431971
GTATAAT6900.023.3005831
GTTTCAC34900.022.8986823
GTTCGGA1600.002077681522.837221
TAATACT6350.022.6737564
Produced by FastQC (version 0.11.5)