FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373556
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.045.544298
GTCTCGC9800.038.312131
GTCTTGC21250.033.9515271
TCGCTCT10650.031.7686144
CGCTCTG11300.030.5782625
CTCGCTC11150.029.6977713
GTTAGCC12750.029.3585113
TCTCGCT12850.028.0096552
GTATTAG7750.027.54791
GTATTAA8650.027.2349171
CTGTCGC17450.027.2280679
GTGTTAG17200.026.537221
TGTTAGC15650.026.2181112
GTATAAT8000.024.8465421
GTCTCAC27600.023.7395861
GTGTAAT9650.023.6497521
GTGTTAA10400.023.3599971
GTCCTAT7250.023.3551161
GGGTAAC7150.022.6521191
ACCGTGT7100.022.3065628
Produced by FastQC (version 0.11.5)