FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887236
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.037.6935358
GTCTCGC12600.035.123461
CTGTCGC13400.034.9203879
GTATAAC4900.032.573511
GTCTTAC10100.028.7327081
GTCTTGC26500.028.1987151
TCGCTCT14150.027.5131344
GTGTTAG19550.027.4614141
GTTAGCC15400.027.1539253
GTGTTAT10950.026.5023141
TCTCGCT16750.025.3962782
GTATTAT8650.024.3231531
GTATTAG10400.023.7182981
CTCGCTC16250.023.5151543
TGTTAGC19200.023.2821622
GTATATA11750.022.2281131
GTATAAT9700.021.6902331
GTCTCAC32000.021.538311
TTGCTCT37000.021.4335294
CGCTCTG16000.021.1488115
Produced by FastQC (version 0.11.5)