FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931650
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.042.117138
GTCTCGC12000.033.4228971
TCGCTCT15200.029.3480664
CTGTCGC18450.028.469039
GTCTTGC25150.026.6754931
CTCGCTC15950.026.1636713
GTACCGA1450.001186119625.1457521
TCTCGCT15250.024.5338022
TCCGTAC2351.4232046E-624.4936523
CGCTCTG16500.024.4194285
GTATTAT8200.024.0111261
GTATAAT7400.022.6651571
GTCTAGC12300.022.528961
GTGTTAG18900.022.378391
GTATTAA6900.022.193861
GTGTATA12650.021.9056261
CGTTATT2303.3350232E-521.8978562
GTGCGTA2353.5495166E-521.721651
GTGTTAT10900.021.4084951
GTATTAG9900.021.361191
Produced by FastQC (version 0.11.5)