FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931650
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12150.034.9656228
GTCTCGC12500.031.7853971
TCGCTCT14950.027.4526564
GTCTTGC23450.026.9835761
GTATAAG8050.025.5921061
CTGTCGC18350.023.9364289
GTATAAC4700.023.482121
TAATACG1550.001883179423.2267824
CGCTCTG14750.022.9433365
GTCTAGC10800.022.4819551
GCGTTAC2002.8102708E-422.0731931
TCTCGCT15900.021.740842
TTCGCTC4659.094947E-1221.6783283
CTCGCTC14650.021.6254793
GTTTCGC5800.021.5657631
CTGTACG2704.7229732E-621.3342294
TTATACT8100.021.3342274
TAATACT7450.021.2626364
GTCTTAC12650.020.9389951
CTCGATC11100.020.7583358
Produced by FastQC (version 0.11.5)