FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540529
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGTGGATATCTCGTATGCCGT39950.25932650407749547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG951.0728134E-437.889549
GTACGTA850.00273614534.1336361
GCGTTAG1352.1360842E-532.2373241
GTCTTAC5500.030.3323921
TCGCTCT7250.029.8258084
TCTGTCG5950.029.0370948
GTCTCGC7450.028.2347031
CTCGCTC7600.027.5047363
GTACTAA2654.5401976E-927.3713131
TACCCGT1607.165053E-526.9971755
TAACGTT1100.00995164226.2105584
GTGTTAG9450.026.0968821
CCGTGCG1701.0823251E-425.4082819
CTGTCGC7450.025.1240739
GTGTAAC3501.2914825E-1024.8687931
GTCTTGC14000.024.3506931
TCTCGCT8300.024.316612
GTGTAAG6750.023.6407051
GTATTAA4303.6379788E-1223.6157151
GTCTAAC2852.7682472E-722.9054661
Produced by FastQC (version 0.11.5)