FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540529
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6200.032.5141948
ACTTTCG1352.2476932E-531.9980958
ACCGGTT1153.2950303E-431.3024868
GTCTCGC5950.029.3153021
GTCTTGC13500.028.5326581
GTCGGAT1050.00761823927.6866741
GTACAGG6950.025.0972751
GTCTAAC3501.2551027E-1024.9180091
GTATAGC2802.2896165E-723.3606341
TCGCTCT7600.022.7347494
GTCCGGT1600.002144965822.7117251
GTCTTAC6100.022.6372621
GTATTAA3604.7184585E-922.2070221
GTATAAA5001.8189894E-1221.8032591
TAGTACT2654.019221E-621.733854
GGAGTGC32700.021.3558355
TAGCCGT1700.003221941621.1745224
GTATTAG3457.3192496E-821.0659481
GTATATA5201.8189894E-1220.964671
GGGTTTC24800.020.8068711
Produced by FastQC (version 0.11.5)